Effect of N-linked glycosylation at position 162 of hemagglutinin in influenza A virus A(H1N1)pdm09

Subtype H1N1 influenza A virus (IAV) that caused a pandemic in 2009, called A(H1N1)pdm09, has been circulating as seasonal IAV among humans, escaping from herd immunity through amino acid substitutions hemagglutinin (HA). Additionally, position 162 of HA A(H1N1)pdm09 evolved to be N-linked glycosylated by 2017. Here the effect glycosylation at was examined comparing ratio nonsynonymous diversity (πN) synonymous (πS) (πN/πS) structurally defined categories sites between 1785 (162+) and 7562 (162−) sequences were not considered 162, respectively. In 162− sequences, πN/πS for located inside 15 Å significantly smaller than one greater outside Å. 162+ corresponding values different each other. These results suggest may shield epitopes against antibodies A(H1N1)pdm09.